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Autometically tune the gains while manual tuning the programmer set all the gains manually. Manual Tuning is just like hit & Trial Process. Standard Spectra tune has ratio targets for it to achieve for the specific ions that it is looking for. This means that the Autotune has a minimum target but no maximum where as the Standard Spectra Tune has a minimum and a maximum for the ions it is looking for. Let’s look at the Tune Wizard for each tune and see what can be. LC-MS Calibration vs. There are two procedures that are used to make sure that an HPLC equipped with a mass spectral detector (MS) is reporting proper mass information and that the system is properly set to detect the analyte(s) of interest. These are calibration and tuning – two processes that are sometimes confused. Manual Tuning with a Long Dead Time This kind of situation is common with temperature loops because dead time is usually a significant factor. This approach is best used where a recorder is connected to the controller. 
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